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Updated: Aug 9, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
[Temperature dependence of collagen by Raman spectra]
Rui-xin Dong1, Xun-ling Yan, Shan Jiang
1Physics Department of Liaocheng University, Liaocheng 252059, China.
Abstract:
Raman spectra of collagen I at different temperature were obtained. There was no change at 1003 cm(-1) line, 1302 cm(-1) line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 degrees C respectively. The points at 40 and 68 degrees C are in agreement with the experimental data by DSC and SHG. The point at 0 degrees C might be frozen transition; the point at 90 degrees C might be related to the damage of secondary structure. When heated to 150 degrees C, the Raman intensity of all bands decreased rapidly and many lines disappeared.
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