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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
Recombinant protein secretion in Escherichia coli
F J M Mergulhão1, D K Summers, G A Monteiro
1Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, Lisbon 1049-001, Portugal. filipem@alfa.ist.utl.pt
Biotechnology Advances
|March 15, 2005
Summary
Secretory production of recombinant proteins in Escherichia coli offers advantages over intracellular methods. This review explores strategies for efficient protein secretion, improving yield and reducing costs.
Area of Science:
- Biotechnology
- Microbiology
- Molecular Biology
Background:
- Intracellular production of recombinant proteins in Escherichia coli often results in inclusion bodies, complicating downstream processing.
- Secretory production targets proteins to the periplasmic space or culture medium, aiding solubility and biological activity.
- This approach can significantly reduce overall process costs for recombinant protein manufacturing.
Purpose of the Study:
- To review various strategies for achieving secretory production of recombinant proteins in Escherichia coli.
- To discuss the advantages and limitations of each secretion strategy.
- To guide the selection of appropriate methods based on target protein characteristics.
Main Methods:
- Literature review of established and emerging recombinant protein secretion systems in E. coli.
- Analysis of different protein targeting signals and secretion pathways.
- Evaluation of factors influencing secretion efficiency, protein folding, and stability.
Main Results:
- Several effective strategies exist for directing recombinant proteins to the periplasm or extracellular medium in E. coli.
- Each strategy presents unique benefits and drawbacks concerning protein yield, folding, and purification.
- The choice of strategy is highly dependent on the specific properties of the recombinant protein.
Conclusions:
- Secretory production in E. coli is a viable and often superior alternative to intracellular production for soluble, active recombinant proteins.
- Optimized secretion strategies can enhance downstream processing, reduce costs, and improve product quality.
- Further research into novel secretion systems can further advance recombinant protein manufacturing in E. coli.
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