Related Experiment Video
Updated: Aug 19, 2026
Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
Influence of Sambucus nigra bark lectin on cell DNA under different in vitro conditions
Larysa L Macewicz1, Olena M Suchorada, Lyubov L Lukash
1Department of Human Genetics, Institute of Molecular Biology and Genetics, NAS of Ukraine, Zabolotnoho street, 150, Kyiv 03143, Ukraine.
Abstract:
The biological activity of Sambucus nigra bark lectin on Chinese hamster cells in vitro was investigated by comet-assay and cytotoxicity testing. Mitogenic properties at the concentrations 0.063-0.25 microg/ml (but not higher) were found, and the induction of DNA breaks at concentrations 0.5 microg/ml and higher is demonstrated. S. nigra bark lectin at mitogenic concentrations decreased the level of nickel-induced DNA damage. The character and mechanism of this lectin protective activity was probably related to the induction of DNA reparation in the cells, decreasing nickel uptake in cells, and non-specific binding of nickel ions by protein molecules.
