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Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
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Small antibiotic resistance plasmids in Staphylococcus intermedius.

R T Greene1, S Schwarz

  • 1Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen.

Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|February 1, 1992
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Summary

This study analyzed Staphylococcus intermedius from dogs in Germany and the USA, finding plasmid-positive isolates in both countries. These plasmids confer resistance to common antibiotics like chloramphenicol, macrolides, and tetracycline.

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Area of Science:

  • Veterinary Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Staphylococcus intermedius is a common canine pathogen.
  • Antibiotic resistance in veterinary pathogens is a growing concern.
  • Plasmids are key mobile genetic elements mediating antibiotic resistance.

Purpose of the Study:

  • To characterize antibiotic resistance plasmids in Staphylococcus intermedius isolates from dogs.
  • To compare plasmid profiles between isolates from Germany and the USA.
  • To investigate the genetic relatedness of these resistance plasmids to known staphylococcal plasmids.

Main Methods:

  • Isolation and identification of Staphylococcus intermedius from canine samples.
  • Determination of antibiograms and plasmid DNA profiles.
  • Plasmid characterization using protoplast transformation and restriction endonuclease analysis.
  • Construction of restriction maps and comparative analysis with reference plasmids.

Main Results:

  • Plasmid DNA was detected in 34.6% of German and 22.2% of USA S. intermedius isolates.
  • Eight small plasmids (2.5–4.5 kb) encoding resistance to chloramphenicol, macrolides, and tetracycline were identified.
  • Restriction mapping revealed structural homologies with plasmids from human-origin Staphylococcus aureus.

Conclusions:

  • Plasmids carrying antibiotic resistance genes are prevalent in canine Staphylococcus intermedius populations in both Germany and the USA.
  • The identified resistance plasmids share structural similarities with those found in human-associated staphylococci, suggesting potential horizontal gene transfer.
  • This highlights the importance of monitoring antibiotic resistance in companion animals.