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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
Identification of two-component regulatory genes involved in o-xylene degradation by Rhodococcus sp. strain DK17
Dockyu Kim1, Jong-Chan Chae, Gerben J Zylstra
1Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea.
Abstract:
Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
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