Related Experiment Video
Updated: Aug 19, 2026

A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats
Published on: November 6, 2018
Phospholipase C gamma in distinct regions of the ventral tegmental area differentially regulates morphine-induced
Carlos A Bolaños1, Rachael L Neve, Eric J Nestler
1Department of Psychology and Program in Neuroscience, Florida State University, Tallahassee, Florida 32306-1270, USA. bolanos@psy.fsu.edu
Abstract:
Neurotrophic factors and the signaling pathways they activate play a role in mediating long-term molecular, cellular, and behavioral adaptations associated with drug addiction. Here we mimicked the biological response of phospholipase C-gamma (PLC gamma) induction in the ventral tegmental area (VTA) observed after chronic morphine using viral-mediated gene transfer. Using a behavioral sensitization paradigm, we demonstrate that microinjections of PLC gamma 1 into distinct (rostral vs. caudal) regions of the VTA result in differential locomotor responses to morphine.
More Related Videos
Related Concept Videos
Analgesia and Pain Management
IP3/DAG Signaling Pathway

