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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
Published on: December 23, 2016
[Specific humoral immune response in Rhesus monkeys vaccinated immune response with Abeta42 peptide vaccine]
Shao-bing Li1, Hua-qiao Wang, Xian Lin
1Department of Anatomy, Zhongshan Medical College, SUN Yat-Sen University, Guangzhou 510080, China.
Aim:
To observe the humoral immuneresponse in Rhesus monkey induced by Abeta42 peptide vaccination.
Methods:
Five male Rhesus monkeys were received intramuscular injection of Abeta42 peptide vaccine at 0, 2nd, 6th, 10th, 14th, 18th, 22th week. The titers and Ig subclasses of the serum anti-Abeta42 antibody were measured by ELISA. The specificity of the anti-Abeta42 antibody was determined by Western blot. The recognition of Abeta plaques in Tg2576-transgenic mouse brain tissue by anti-Abeta42 serum were detected by immunohistochemical staining.
Results:
At the 8th week after the vaccination, the serum anti-Abeta42 antibody was detected. The titers of the antibody increased with times of booster inoculation and reached 1:4,320 at the 24th week, then decreased. The produced antibodies were mainly IgG1 and IgG2(IgG2/IgG1>1). The anti-Abeta42 antibody had high specificity. The Abeta plaques in Tg2576-transgenic mouse brain tissue were recognized by the antisera.
Conclusion:
Abeta42 peptide vaccine can induce effectively specific humoral immuneresponse in Rhesus monkey.
