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Updated: Aug 19, 2026
Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
Published on: September 25, 2019
[Immunopathology of hepatitis B virus transgenic mouse strain C57-TgN (adr2.0) SMMU]
1Department of Pathology, Changzheng Hospital, Shanghai 200003, China.
Aim:
To make immunopathological study of hepatitis B virus transgenic mice line C57-TgN (HBV adr2.0) SMMU.
Methods:
Twenty transgenic C57-TgN mice of SPF grade and corresponding control mice C57BL/6 were used for the study. The expressions of CD3, CD4 and CD8 on lymphocytes in peripheral blood from transgenic and normal C57BL/6 mice were detected by flow cytometry. T lymphocyte subsets in the liver tissues from transgenic mice and hepatitis B patients were analyzed by EnVision staining.
Results:
The expression of CD3, CD4 and CD8 on lymphocytes in peripheral blood from transgenic mice was lower than those from normal mice. The infiltrative mononuclear cells in the liver tissues of transgenic mice were mainly CD3+ CD4+ cells, and no CD8+ CD57+ cells was found. The infiltrative mononuclear cells in the liver tissues of human hepatitis B patients were mainly CD3+ CD4+ or CD3+ CD8+ cells.
Conclusion:
T cell subsets in the peripheral blood and liver tissues of transgenic mice C57-TgN (adr2.0) SMMU are different from those in human chronic hepatitis B patients.
