Related Experiment Video

Updated: Aug 19, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
06:19

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay

Published on: May 12, 2020

[Malondialdehyde and reduced glutathione increase in patients with chronic stable ischaemic cardiopathy]

Mayela Carolina Cabrera de Bravo1, Valmore José Bermúdez Pirela, Fernando Bermúdez Arias

  • 1Centro de Investigaciones Endocrino-metabólicas Dr. Félix Gómez, Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.

Medicina Clinica
|March 16, 2005
PubMed

Insights

Chronic ischemic heart disease impacts the oxidation/antioxidation balance. This study found elevated malondialdehyde and glutathione levels in patients, suggesting their potential as metabolic indicators for monitoring disease progression and tissue damage.

Area of Science:

  • Biochemistry
  • Cardiology
  • Oxidative Stress Research

Background:

  • Cardiovascular diseases are linked to ischemia/reperfusion, affecting the oxidation/antioxidation balance.
  • Understanding this balance is crucial for managing chronic ischemic heart disease.

Purpose of the Study:

  • To investigate the oxidation/antioxidation status in patients with chronic ischemic heart disease.
  • Specifically, to measure malondialdehyde, nitric oxide, glutathione, ascorbic acid, and dehydroascorbic acid levels.

Main Methods:

  • Study included 32 male patients (40-60 years) with chronic ischemic heart disease, divided into hypertensive and non-hypertensive groups.
  • A control group of 31 healthy males was used for comparison.
  • Biochemical markers related to oxidative stress were analyzed.

Main Results:

  • Significantly higher malondialdehyde levels were found in both ischemic groups compared to the healthy control group (p < 0.001).
  • The hypertension ischemic group exhibited significantly greater reduced glutathione levels compared to the control (p < 0.03) and non-hypertension ischemic groups (p < 0.02).
  • No significant differences were observed for other measured parameters across the groups.

Conclusions:

  • The oxidation/antioxidation balance serves as a valuable metabolic indicator for ischemia in chronic ischemic heart disease.
  • Monitoring these markers can aid in evaluating therapeutic effectiveness and detecting early molecular changes indicative of tissue damage.
Abstract

More Related Videos

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Related Concept Videos

Atherosclerosis III: Management01:26

Atherosclerosis III: Management

Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...