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Related Experiment Videos

Assessing analytical specificity in quantitative analysis using tandem mass spectrometry.

Mark M Kushnir1, Alan L Rockwood, Gordon J Nelson

  • 1ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA. kushnmm@aruplab.com

Clinical Biochemistry
|March 16, 2005
PubMed
Summary

Confirming compound identity is crucial in quantitative analysis, even with multiple-stage mass spectrometry (MSn). This study introduces a method using multiple mass transitions and branching ratios to ensure analytical specificity and accurate results.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Compound identity confirmation is vital in quantitative analysis, especially with mass spectrometry.
  • Established rules for assessing analytical specificity in multiple-stage mass spectrometry (MSn) are lacking.

Purpose of the Study:

  • To establish rules for assessing analytical specificity in quantitative MSn analyses.
  • To propose a method for confirming compound identity in MSn-based quantitative analysis.

Main Methods:

  • Evaluated approaches for assuring compound identity.
  • Monitored multiple mass transitions of a target compound.
  • Compared branching ratios of mass transitions.

Main Results:

  • Monitoring multiple mass transitions and their intensity ratios can distinguish target analytes from interferences.

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  • The proposed strategy and acceptance criteria are specific to the compound and method.
  • Criteria should be established during method development and validation.

    • Conclusions:

    • Certainty of analyte identity is paramount in quantitative MSn analysis.
    • Methods to verify analyte identity are essential for critical applications.
    • The developed approach enhances the reliability of quantitative MSn results.