Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cost-effective production of Bacillus thuringiensis by solid-state fermentation.

P S Vimala Devi1, T Ravinder, C Jaidev

  • 1Directorate of Oilseeds Research, Rajendranagar, Hyderabad 500 030, Andhra Pradesh, India. psvdevi@yahoo.co.in

Journal of Invertebrate Pathology
|March 16, 2005
PubMed
Summary

This study optimized Bacillus thuringiensis (Bt) production for effective pest control. The developed method significantly reduced castor semilooper populations in field trials.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factor analysis validation of the student feedback questionnaire for the subcutaneous swelling simulation model.

Journal of education and health promotion·2026
Same author

Hospital-based sentinel surveillance for bacterial meningitis in under-five children prior to the introduction of the PCV13 in India.

Vaccine·2021
Same author

Novel formulations of Bacillus thuringiensis var. kurstaki: an eco-friendly approach for management of lepidopteran pests.

World journal of microbiology & biotechnology·2020
Same author

Characterization and regulation of Bacillus thuringiensis Cry toxin binding aminopeptidases N (APNs) from non-gut visceral tissues, Malpighian tubule and salivary gland: Comparison with midgut-specific APN in the moth Achaea janata.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology·2013
Same author

Cloning, characterization, and expression of a new cry1Ab gene from DOR Bt-1, an indigenous isolate of Bacillus thuringiensis.

Molecular biotechnology·2012
Same author

Characterization of Bacillus thuringiensis strain DOR4 toxic to castor semilooper Achaea janata: proteolytic processing and binding of toxins to receptors.

Current microbiology·2008

Area of Science:

  • Agricultural Entomology
  • Microbial Pest Control

Background:

  • Bacillus thuringiensis (Bt) is a bacterium used as a biopesticide.
  • Optimizing Bt production is crucial for cost-effective pest management.

Purpose of the Study:

  • To standardize and scale-up Bacillus thuringiensis (Bt) production.
  • To evaluate the efficacy of produced Bt against castor semilooper (Achaea janata L).

Main Methods:

  • Standardized Bt production on wheat bran-based media in Erlenmeyer flasks.
  • Scaled-up production in plastic tubs with controlled aeration.
  • Incubation at 30°C for optimal cell lysis (60 hours).
  • Laboratory bioassays and field trials to assess efficacy.

Main Results:

Related Experiment Videos

  • Scale-up significantly increased Bt spore count and toxin content.
  • Optimal lysis achieved at 60 hours of incubation at 30°C.
  • Bt demonstrated high efficacy at 0.1% concentration, causing complete mortality of Achaea janata L. larvae within three days.
  • Field trials showed significant reduction in castor semilooper populations.

Conclusions:

  • The developed protocol is suitable for producing effective Bt strains and local isolates.
  • Bt biopesticide offers a cost-effective solution (approx. $0.70/kg) for managing castor semilooper infestations.
  • This research supports sustainable agriculture through microbial pest control.