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Development of an enzyme-linked immunosorbent assay (ELISA) for the specific detection of MEKK1 expression in cells
Yan Ding1, Dan Huang, Xiao-Guang Chen
1Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, China.
Introduction:
MEKK1 is a 196-kDa serine-threonine kinase activated in response to a variety of stimuli, including EGF, lysophosphatidic acid, osmotic stress, UV light, and microtubule toxins. However, there are few reports about the expression level of MEKK1 in cancers.
Methods:
In this report, a direct competitive ELISA to quantify total MEKK1 in cell lines was developed.
Results:
The procedure showed a high sensitivity (detection limit: 0.17 ng/ml), good precision (coefficient of variation
Discussion:
This method can be used to measure the MEKK1 level of cell samples. The activity of MEKK1/JNK pathway in pancreas cancer and umbilical vein cell lines indicates that MEKK1 may be a potential target in interfering with pancreas cancer and angiogenesis.
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