Related Experiment Video
Updated: Aug 19, 2026

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
[Study on fluorescence characteristics of pefloxacin based on charge transfer reaction]
1College of Chemistry, Yantai University, Yantai 264005, China.
Abstract:
The charge transfer complex formed between pefloxacin mesylate (PM) as the donor and tetrachlorobenzoquinone (TCQ) as the acceptor has been studied by fluorimetry. Experiment shows that PM reacted with TCQ in methanol with light and the complex was formed. Its fluorimetric intensity was stronger than that of PM and the excitation and emission peaks of the complex were red shifted to 80 nm and 60 nm, respectively. A linear calibration graph was obtained over the PM concentration from 0.05 micromol x L(-1) to 10 micromol x L(-1) with the detection limit of 0.004 micromol x L(-1) of PM. The proposed method and UV-Vis photometric method were both applied to determining the pefloxacin in the same capsules with satisfactory results.
Related Concept Videos
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Applications

