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[Determination of deoxyribonucleic acid with nitro-tetrazolium chloride blue by resonance light-scattering technique]

Yun-fei Long1, Xiao-ming Chen, Tie-jun Cai

  • 1Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|March 17, 2005
PubMed

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...