Related Experiment Video

Updated: Aug 19, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

Multiple signaling is involved in endostatin-mediated apoptosis in ECV 304 endothelial cells

Bin Ren1, Yuan Wang, Kenneth Ndebele

  • 1School of Life Science, University of Science and Technology of China, Hefei 230027, China. bin_ren@hms.harvard.edu <bin_ren@hms.harvard.edu>

Frontiers in Bioscience : a Journal and Virtual Library
|March 17, 2005
PubMed

Insights

Endostatin induces programmed cell death (apoptosis) in endothelial cells via a caspase-dependent pathway. This anti-angiogenic mechanism involves key signaling molecules like p53 and Bcl-2.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Apoptosis of vascular endothelial cells is crucial for regulating angiogenesis.
  • Endostatin is a promising anti-angiogenic therapeutic, but its precise effects on endothelial cell apoptosis pathways are not fully understood.

Purpose of the Study:

  • To investigate the effects of human endostatin on endothelial cell apoptosis.
  • To elucidate the specific molecular mechanisms and signaling pathways involved in endostatin-induced apoptosis.

Main Methods:

  • Expression of human endostatin in E. coli.
  • Treatment of ECV 304 endothelial cells with expressed endostatin.
  • Assessment of cell viability, apoptosis, and key signaling molecules (caspases, p53, Bcl-2, intracellular ions).

Main Results:

  • Expressed human endostatin reduced ECV 304 cell viability in a dose-dependent manner.
  • Endostatin triggered apoptosis through a caspase-dependent mechanism.
  • Apoptosis was linked to intracellular magnesium and calcium overload and altered p53 and Bcl-2 expression.

Conclusions:

  • Human endostatin effectively induces apoptosis in endothelial cells.
  • The process is mediated by caspases and involves significant alterations in apoptotic signaling pathways.
  • Findings provide insight into endostatin's anti-angiogenic potential at the cellular level.

More Related Videos

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells11:56

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

Published on: May 15, 2014

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...