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Serum calcium-decreasing factor (caldecrin) from porcine pancreas has proteolytic activity which has no clear

A Tomomura1, T Fukushige, T Noda

  • 1Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.

FEBS Letters
|April 27, 1992
PubMed
Summary
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Researchers purified a novel pancreatic factor that lowers serum calcium levels. This factor inhibits parathyroid hormone (PTH) action on bones, suggesting a new mechanism for calcium regulation.

Area of Science:

  • Biochemistry
  • Endocrinology
  • Pharmacology

Background:

  • Serum calcium homeostasis is critical for physiological functions.
  • Parathyroid hormone (PTH) is a key regulator of serum calcium, primarily acting on bone.
  • Pancreatic extracts have been investigated for potential roles in metabolic regulation.

Purpose of the Study:

  • To purify and characterize a novel factor from porcine pancreas with serum calcium-lowering activity.
  • To investigate the mechanism of action of this factor, particularly its interaction with parathyroid hormone (PTH).

Main Methods:

  • Purification of the factor to homogeneity from porcine pancreas.
  • In vivo studies in mice and rats to assess serum calcium and hydroxyproline levels.
  • In vitro studies using cultured fetal long bone to evaluate inhibition of 45Ca release stimulated by various agents, including PTH and PTH-related protein.

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Main Results:

  • A factor with chymotrypsin-like protease activity was purified.
  • Intravenous administration of the factor decreased serum calcium in mice and rats.
  • The factor inhibited 45Ca release from bone stimulated by PTH and PTH-related protein but not by other bone-active agents.
  • Treatment with PMSF, a serine protease inhibitor, enhanced the factor's activity.

Conclusions:

  • The purified factor possesses significant serum calcium-decreasing activity.
  • The mechanism likely involves inhibition of PTH action on bone, independent of direct proteolytic degradation.
  • This suggests a novel pathway for calcium regulation potentially mediated by pancreatic factors.