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Inserting foreign peptides into the major coat protein of bacteriophage M13
A A Ilyichev1, O O Minenkova, G P Kishchenko
1Research and Technology Institute of Biologically Active Substances, Vektor NPO, Berdsk, Novosibirsk, Region, USSR.
FEBS Letters
|April 27, 1992
Abstract:
Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B-proteins have the same contour length and the same number of B-protein molecules as virions with natural B-proteins. It was shown that for one of hybrid B-proteins the position of the processing site had changed.