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Updated: Aug 19, 2026
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
Published on: December 28, 2015
[High cell density fed-batch culture of E.coli expressing rhVEGF121]
Zhi-Ming Hu1, Li Ma, Ming-Qian Zhou
1Institute of Molecular Immunology, Southern Medical University, Guangzhou 510515, China. hzm@fimmu.com
Objective:
To investigate the optimal high cell density fermentation conditions of recombinant E.coli BL21/pET- 24a/hVEGF(121) expressing recombinant human vascular endothelial growth factor (rhVEGF(121)).
Methods:
The effects of the composition of the fermentation medium, induction time and fed-batch carbon sources on the expression level of rhVEGF(121) and cell output were analyzed.
Results And Conclusion:
When cultured in modified M9 medium and induced for 4 h in the presence of 0.5 mmol/L IPTG at 37 degrees celsius; with glycerol as the carbon sources by continuous fed-batch mode, the recombinant E.coli expressed rhVEGF(121) at the level up to 23% of the total proteins and the yield reached 68 g/L. The optimized fermentation condition for recombinant E.coli enables high expression level of rhVEGF(121).
