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Arcobacter cibarius sp. nov., isolated from broiler carcasses
Kurt Houf1, Stephen L W On2, Tom Coenye3
1Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Summary
Twenty novel Arcobacter isolates from broiler carcasses represent a new species, Arcobacter cibarius. These bacteria were identified using molecular and biochemical methods, distinguishing them from known Arcobacter species.
Area of Science:
- Microbiology
- Bacteriology
- Food Safety
Background:
- Arcobacter species are emerging foodborne pathogens.
- Broiler carcasses are a potential reservoir for Arcobacter.
Purpose of the Study:
- To characterize and identify novel Arcobacter isolates from broiler carcasses.
- To determine if these isolates represent a new species.
Main Methods:
- Polymerase chain reaction (PCR) for Arcobacter genus and species identification.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for whole-cell protein analysis.
- DNA-DNA hybridization and 16S rRNA gene sequencing for phylogenetic analysis.
- Biochemical tests for phenotypic characterization.
Main Results:
- Twenty isolates were identified as Arcobacter but distinct from known species.
- SDS-PAGE, DNA-DNA hybridization, and 16S rRNA gene sequencing supported a novel species designation.
- Isolates showed unique biochemical profiles differentiating them from other Arcobacter species.
- The novel species, Arcobacter cibarius sp. nov., was proposed with LMG 21996(T) as the type strain.
Conclusions:
- The 20 isolates represent a single, novel Arcobacter species, Arcobacter cibarius.
- This finding expands the known diversity of Arcobacter and highlights potential reservoirs in poultry.
- Arcobacter cibarius can be differentiated from other Arcobacter species using specific biochemical tests.