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Updated: Aug 19, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
Published on: January 27, 2023
[Differentiation of osteoclasts]
1Department of Oral Histology, Showa University School of Dentistry.
Abstract:
The differentiation and functions of osteoclasts (OCs) are regulated by osteoblast-derived factors such as receptor activator of NF kappa; B ligand (RANKL) that stimulates OC formation and a novel secreted member of the TNF receptor superfamily, osteoprotegerin (OPG), that negatively regulates osteoclastogenesis. Preosteoclasts (pOCs) treated with RANKL became TRAP-positive OCs, which express vacuolar-type H (+) -ATPase and exhibit resorptive activity. Such effects of RANKL on pOCs are inhibited by OPG. OPG inhibits ruffled border formation in mature OCs, reduces their resorptive activity, and induces apoptosis.
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