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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

ERK2 is required for FGF1-induced JNK1 phosphorylation in Xenopus oocyte expressing FGF receptor 1

Edith Browaeys-Poly1, Véronique Fafeur, Jean Pierre Vilain

  • 1Laboratoire de Biologie du Développement, Université des Sciences et Technologies de Lille, UPRES EA 1033, IFR 118, Bâtiment SN3, 59655 Villeneuve D'Ascq Cedex, France.

Biochimica Et Biophysica Acta
|March 22, 2005
PubMed

Insights

The study investigated the link between ERK2 and JNK1 MAP kinases in Xenopus oocytes. Results show JNK1 activation is dependent on ERK2 in Fibroblast Growth Factor Receptor 1 (FGFR1) signaling.

Area of Science:

  • Cellular signaling pathways
  • Molecular biology
  • Signal transduction cascades

Background:

  • Fibroblast Growth Factor Receptor 1 (FGFR1) signaling is crucial for cellular processes.
  • Mitogen-activated protein (MAP) kinases, including ERK2 and JNK1, play key roles in signal transduction.
  • Understanding the interplay between different MAP kinase pathways is essential for deciphering cellular responses.

Purpose of the Study:

  • To investigate the potential connection between ERK2 and JNK1 MAP kinase pathways.
  • To elucidate the role of these kinases in Fibroblast Growth Factor 1 (FGF1)-induced signaling in Xenopus oocytes.

Main Methods:

  • Xenopus oocytes expressing FGFR1 were stimulated with FGF1.
  • Inhibitors targeting the Shc/Grb2/Ras/Mos/MEK/ERK2 cascade were injected.
  • Phosphorylation levels of ERK2 and JNK1 were assessed.
  • The effect of active ERK2 and ERK2 inhibition on JNK1 phosphorylation was examined.

Main Results:

  • Inhibition of the ERK2 cascade blocked FGF1-induced germinal vesicle breakdown (GVBD).
  • ERK2 and JNK1 phosphorylation were inhibited by blocking the ERK2 cascade.
  • JNK1 activation was found to be downstream of ERK2.
  • Active ERK2 induced JNK1 phosphorylation, while ERK2 inhibition blocked it.

Conclusions:

  • FGF1-induced GVBD in Xenopus oocytes involves the ERK2 and JNK1 signaling pathways.
  • JNK1 phosphorylation is dependent on ERK2 activity within the FGFR1 signaling pathway.

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