Evidence for bound antineuronal antibodies in brains of NZB/W mice

Antibodies reactive with neuronal tissue are present in the sera of the murine models of systemic lupus erythematosus (SLE). Access of these antibodies to the central nervous system is an important prerequisite to the hypothesis that these antibodies affect neuronal function. In this study, we isolated antibodies from neutral and acid washes of brain parenchyma of NZB/W F1 mice. Antibody could be eluted from the brains of NZB/W F1 but not control mice. The immunoglobulin was predominantly IgG1; the binding characteristics of the brain eluted antibody were narrower than those of antibody from sera and eluted from visceral organ.