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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
Published on: October 8, 2014
Fluorescent metal ion indicators based on benzoannelated crown systems: a green fluorescent indicator for
Vladimir V Martin1, Anca Rothe, Kyle R Gee
1Molecular Probes Invitrogen Detection Technologies, 29851 Willow Creek Road, Eugene, OR 97402, USA.
Abstract:
The synthesis and metal binding properties of cation-sensitive fluorescent indicators intended for biological applications are described. The increase of the crown ether ring size enhances the affinity for larger cations, but weakens the fluorescent response and selectivity. A compound having a 15-crown-5 chelator directly attached to a 2,7-difluoroxanthenone fluorophore loads into live cells and responds to sodium ion concentration changes with large fluorescence increases in the visible wavelength range.
