Related Experiment Video
Updated: Aug 19, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
Published on: November 2, 2018
IFSERF, an isotope-filtered SERF experiment for the precise measurement of proton-proton coupling constants between
Pau Nolis1, Anna Roglans, Teodor Parella
1Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Barcelona, Spain.
Abstract:
An isotope-filtered selective refocusing (IFSERF) experiment is presented for the sensitive and precise measurement of the proton-proton coupling constant between chemically equivalent protons. The 2D NMR method combines an initial doubly selective isotope filter based on heteronuclear cross-polarization followed by a selective J-resolved block. The coupling topologies obtained from several 2D variants of the IFSERF experiment are described for the simultaneous measurement of both proton-proton and proton-carbon coupling constants in the involved AA'XX' spin system. Application on the determination of the relative configuration of double bonds in symmetrical molecules is illustrated.
More Related Videos
11:44Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes
Published on: November 12, 2016
14:55Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
Related Concept Videos
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Chemical Shift: Internal References and Solvent Effects
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Spin–Spin Coupling Constant: Overview
Qualitatively, any spin plus-half nucleus polarizes the spins of its electrons to the minus-half state. Consequently, the paired electron in the hydrogen–carbon bond must have a...
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹H NMR: Complex Splitting
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei in a...