Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Selenium binding to human hemoglobin via selenotrisulfide.

Mamoru Haratake¹, Katsuyoshi Fujimoto, Masahiro Ono

¹Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. haratake@net.nagasaki-u.ac.jp

Biochimica Et Biophysica Acta

|March 23, 2005

Summary

A stable selenotrisulfide, PenSSeSPen, reacts with hemoglobin (Hb) in red blood cells (RBCs). Selenium binds to Hb

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Improving Pharmacokinetics of PSMA/GRPR-Targeting Radiolabeled Heterodimer through Enhanced Albumin Binding.

Molecular pharmaceutics·2026

Same author

Bithiophene Scaffold for PET Imaging and Photosensitization as a Novel Theranostic Platform Targeting Amyloid-β Aggregates.

ACS chemical neuroscience·2026

Same author

Systematic Investigation of Fatty Acids with Different Carbon-Chain Length as Albumin Binder to Improve Tumor Accumulation of Radioligands.

Bioconjugate chemistry·2026

Same author

Head-to-Head Comparison of Monomeric, Homodimeric, and Heterodimeric Fibroblast Activation Protein-Targeting Radioligands Based on Small Molecule Inhibitor and Cyclic Peptide.

ACS pharmacology & translational science·2026

Same author

Development of Novel Cholecystokinin 2 Receptor-Targeted Radioligand Containing Albumin Binder for Efficient <sup>111</sup>In/<sup>177</sup>Lu Theranostics.

Bioconjugate chemistry·2026

Same author

Engagement of the TCR against an oncolytic virus generates a population of effector CAR T cells with potent antitumor activity.

Science advances·2026

Area of Science:

Biochemistry
Toxicology
Redox Biology

Background:

Selenotrisulfide is a key intermediate in selenite metabolism.
Its reactivity with biological molecules is poorly understood due to instability.
Penicillamine forms a stable selenotrisulfide, PenSSeSPen, for study.

Purpose of the Study:

Investigate selenite metabolic fate in red blood cells (RBCs).
Explore the reaction of stable selenotrisulfide (PenSSeSPen) with human hemoglobin (Hb).

Main Methods:

Langmuir binding analysis to quantify selenium binding to Hb.
Thiol group blockade using iodoacetamide.
MALDI-TOF mass spectrometry to identify selenium binding sites.

Main Results:

Related Experiment Videos

PenSSeSPen rapidly reacts with Hb under physiological conditions.
Selenium binding correlates with the number of reactive Hb thiol groups.
PenSSe moiety binds to beta subunits of Hb, indicating thiol exchange.

Conclusions:

PenSSeSPen effectively models selenotrisulfide reactions with Hb.
The reaction involves thiol exchange between penicillamine and Hb cysteine residues.
This provides insight into selenite metabolism in RBCs.