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Updated: Aug 12, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations
Bhaskar Chaurasia1, Anita Pandey, Lok Man S Palni
1G B Pant Institute of Himalayan Environment and Development Kosi-Katarmal-Almora, 263 643 Uttaranchal, India.
Abstract:
An efficient antagonistic strain of Bacillus subtilis, originally isolated from the rhizosphere of established tea bushes, was found to cause structural deformities in six pathogenic fungi under in vitro culture conditions. This effect was attributed to the production of diffusible and volatile antifungal compounds. Out of the selected test fungi four were phytopathogenic, while the remaining two were of clinical importance. The bacterial strain successfully restricted the growth of all test fungi in dual cultures, and induced morphological abnormalities such as mycelial and conidial deviations. The inhibitory effect caused by volatiles was greater than that by diffusible compounds.
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