Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Base pair opening in three DNA-unwinding elements.

Daniel Coman1, Irina M Russu

  • 1Department of Chemistry and the Molecular Biophysics Program, Wesleyan University, Middletown, Connecticut 06459, USA.

The Journal of Biological Chemistry
|March 24, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution thermal imaging to delineate effects of cancer-induced aerobic glycolysis and endothelial dysfunction.

Neuro-oncology advances·2026
Same author

Overcoming Immune Exclusion in a Murine Model of Cirrhotic Hepatocellular Carcinoma via Cryoablation: Insights from Molecular Extracellular pH Imaging.

Research square·2025
Same author

[<sup>18</sup>F]SynVesT-1 PET Detects SV2A Changes in the Spinal Cord and Brain of Rats with Spinal Cord Injury.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2025
Same author

Cysteine depletion triggers adipose tissue thermogenesis and weight loss.

Nature metabolism·2025
Same author

Intranasal Leukemia Inhibitory Factor as a late-stage treatment for delayed white matter damage in concussive head injury.

bioRxiv : the preprint server for biology·2025
Same author

Molecular MRI of T-cell immune response to cryoablation in immunologically hot <i>vs</i>. cold hepatocellular carcinoma.

JHEP reports : innovation in hepatology·2025
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
Same journal

Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

The Journal of biological chemistry·2026
Same journal

Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

The Journal of biological chemistry·2026
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
See all related articles

This study characterizes individual base pair opening in DNA-unwinding elements crucial for DNA replication. Sequence variations influence the kinetics and energetics of base pair opening, impacting bacterial DNA replication origins.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • DNA-unwinding elements initiate DNA replication by facilitating strand separation.
  • These elements contain conserved base sequences critical for replication origins in bacteria.

Purpose of the Study:

  • To characterize the opening of individual base pairs within DNA-unwinding elements.
  • To investigate how base sequence affects the kinetics and energetics of DNA unwinding.

Main Methods:

  • Studied three 13-mer DNA-unwinding elements from Escherichia coli.
  • Utilized nuclear magnetic resonance (NMR) spectroscopy to measure imino proton exchange rates.
  • Determined rates and equilibrium constants for individual base pair opening at 20°C.

Related Experiment Videos

Main Results:

  • Demonstrated distinct kinetics and energetics for Adenine-Thymine/Thymine-Adenine (AT/TA) base pair opening among the three DNA elements.
  • Identified long-range base sequence effects influencing AT/TA base pair opening.
  • Observed a correlation between AT/TA base pair opening properties and element location within replication origins.

Conclusions:

  • Base sequence context significantly modulates DNA-unwinding element function.
  • Variations in AT/TA base pair opening kinetics and energetics are conserved across bacterial genomes.
  • Element location within replication origins correlates with base pair opening characteristics.