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Related Experiment Videos

Interplay between septin organization, cell cycle and cell shape in yeast.

Amy S Gladfelter1, Lukasz Kozubowski, Trevin R Zyla

  • 1Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.

Journal of Cell Science
|March 24, 2005
PubMed
Summary

Septins are essential cytoskeletal proteins. This study reveals their novel role in bud shaping and identifies new genes involved in septin organization, crucial for cell growth and division.

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Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Genetics

Background:

  • Septins are filament-forming proteins crucial for cell division in eukaryotes.
  • Their precise localization and organization mechanisms remain largely unknown.
  • In Saccharomyces cerevisiae, Cdc42p regulates septin organization at the mother-bud neck.

Purpose of the Study:

  • To investigate the role of septin organization in cell shape determination.
  • To identify novel factors involved in septin cortex assembly.
  • To understand how cell cycle delays impact septin organization.

Main Methods:

  • Analysis of cdc42(V36T,K94E) mutant yeast cells with aberrant septin structures.
  • Microscopy to observe septin localization and cell morphology.

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  • Genetic screening to identify mutants with septin organization defects.

    • Main Results:

    • A well-organized septin cortex is essential for proper bud shaping.
    • Disorganized septins lead to aberrant cell neck formation.
    • Elongated bud shapes exacerbate septin organization defects.
    • Identified CLN1, CLN2, BNI1, BNI4, BUD3, BUD4, and BUD5 as new players in septin assembly.

    Conclusions:

    • Septin cortex organization is critical for directing cell growth and maintaining cell polarity.
    • Septin organization defects can be exacerbated by cell cycle delays, revealing novel genetic interactions.
    • This work expands the known genetic network regulating septin cytoskeleton assembly.