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A new intertype recombinant between genotypes C and D of hepatitis B virus identified in China

Zhanhui Wang1,2, Zhihua Liu1, Guobing Zeng1

  • 1Department of Infectious Diseases, Nanfang Hospital, Guangzhou 510515, China.

The Journal of General Virology
|March 24, 2005
PubMed

Insights

Hepatitis B virus (HBV) isolates initially classified as genotype D were found to be recombinants of genotypes C and D. This intertype recombination increases HBV genetic diversity and may impact diagnosis and treatment.

Area of Science:

  • Hepatology
  • Virology
  • Genetics

Background:

  • Hepatitis B virus (HBV) genotypes exhibit distinct geographical distributions, with genotypes B and C predominant in China.
  • Accurate HBV genotype classification is crucial for understanding disease progression and treatment strategies.

Purpose of the Study:

  • To investigate the genetic characteristics of eight HBV isolates initially classified as genotype D.
  • To determine if intertype recombination has occurred in these HBV isolates and identify recombination sites.

Main Methods:

  • Complete HBV genome sequencing and comparison with reference sequences from genotypes A-G.
  • Phylogenetic analyses of different HBV open reading frames (ORFs), including the S, precore/core, and X genes.
  • Recombination analysis using the simplot program.

Main Results:

  • Phylogenetic analysis of the S gene indicated genotype D, while analyses of other ORFs suggested genotype C.
  • Discordant phylogenetic results across different ORFs indicated intertype recombination in all eight isolates.
  • Recombination with genotype D was localized to the preS2/S region in most isolates.

Conclusions:

  • Intertype recombination is a significant source of genetic diversity in Hepatitis B virus.
  • HBV genotype hybrids may possess unique virological properties requiring further investigation for clinical implications in chronic hepatitis B management.

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