Related Experiment Video
Updated: Aug 18, 2026
Multiplex Cyclic Fluorescent Immunohistochemistry
Published on: January 26, 2024
Immunohistochemical profile of multicentric reticulohistiocytosis
Flávio Barbosa Luz1, Antonio Pedro Gaspar, Marcia Ramos-e-Silva
1Rua Desembargador Izidro, 28/606, 20.521-160, Rio de Janeiro, Brazil. flavioluz@dermatologista.net
Background:
There is enough evidence to support the knowledge that multicentric reticulohistiocytosis (MR) is a histiocytic proliferative disorder; however, the type of histiocytes involved is not well established.
Objective And Methods:
To study the nature of cells present in MR lesions by studying the immunohistochemical profile of three new cases and reviewing 23 cases reported in the literature.
Results:
MR histiocytic cells are positive for vimentin, CD68, and CD45, negative for S-100 protein, CD34, and XIIIa factor, and weak reactors for thrombomodulin. Small activated histiocytes are MAC387 positive. Lymphocytes, mainly CD4+ cells, are found in MR infiltrates.
Conclusions:
The MR immunophenotypic pattern does not suggest a type I or type II dendrocyte or a Langerhans cell origin. On the other hand, it points to a different cell derived from the monocyte-macrophage line. CD4+ cells may be responsible for activating the proliferation of histiocytic cells. Small histiocytic MAC387+ cells are likely to become the MR multinucleated giant cells.
