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Updated: Aug 18, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Precise characterization of the epitope recognized by a monoclonal antibody against Escherichia coli RNA polymerase
Estelle Andre1, Martine Pugniere, Jaqueline Latouche
1CNRS UMR 5160, Faculté de Pharmacie, Montpellier, France.
Abstract:
We have recently isolated a monoclonal antibody directed against Escherichia coli RNA polymerase that does not inhibit transcription. This antibody is a useful tool to immobilize this enzyme for transcription assays or protein-protein interaction studies. The epitope of this monoclonal antibody was precisely located by a combination of protein deletion and synthetic peptide scanning. The amino acids of the epitope were also determined. We conclude that this antibody binds an epitope shared by several bacterial species and therefore can be used to characterize or purify other related enzymes.
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