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GST fusion vector with caspase-6 cleavage site for removal of fusion tag during column purification

Prabhat Kumar Purbey1, P Cyril Jayakumar, P D Deepalakshmi

  • 1National Centre for Cell Science, Ganeshkhind, Pune, India.

Biotechniques
|March 25, 2005
PubMed
Abstract

No abstract available in PubMed .

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Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...