Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oxysterols increase in diabetic rats.

Naoki Yoshioka1, Junko Adachi, Yasuhiro Ueno

  • 1Department of Legal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.

Free Radical Research
|March 25, 2005
PubMed
Summary

Diabetes elevates harmful oxysterols and reduces nitric oxide (NO) in tissues, with kidneys being the primary target for early diabetic complications. This indicates increased oxidative stress contributes to diabetes-related tissue damage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Affordable and Efficient In-House Approach for Detecting Gene Fusions in Bone and Soft Tissue Tumors Using a Custom Capture Panel and Nanopore Sequencing.

Genes, chromosomes & cancer·2026
Same author

Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.

Scientific reports·2026
Same author

MUC4-Positive Fibroblastoma: Clinicopathological and Molecular Analysis of 7 Cases.

The American journal of surgical pathology·2026
Same author

Postmortem blood examination revealed prevalence of heart failure and its comorbidities in bathing-related deaths in older Japanese adults.

Journal of forensic and legal medicine·2026
Same author

Atypical catabolite repression of <i>Geobacillus kaustophilus iol</i> operons in the presence of ribose.

Microbiology (Reading, England)·2026
Same author

Carbon catabolite repression in Gram-positive bacteria, including the recently developed insights.

Current opinion in microbiology·2026

Area of Science:

  • Biochemistry
  • Pathophysiology
  • Toxicology

Background:

  • Diabetes mellitus is associated with increased oxidative stress.
  • Oxidative stress can lead to lipid peroxidation and impaired nitric oxide (NO) signaling.
  • These biochemical changes may contribute to diabetic tissue complications.

Purpose of the Study:

  • To investigate if diabetes enhances lipid peroxidation, evidenced by increased oxysterol levels.
  • To determine if diabetes attenuates nitric oxide (NO) generation.
  • To identify potential target organs for early diabetic complications.

Main Methods:

  • Induction of diabetes in Wistar rats using streptozotocin (STZ).
  • Quantification of various oxysterols (7-hydroxycholesterols, 7-ketocholesterol, 7-hydroperoxycholesterols) using High-Performance Liquid Chromatography (HPLC).

Related Experiment Videos

  • Measurement of NO metabolites (NOx) and blood urea nitrogen (BUN).

    • Main Results:

    • Oxysterol levels were significantly elevated in diabetic rats compared to control rats across kidneys, heart, and liver.
    • The increase in oxysterols was most pronounced in the kidneys, followed by the heart and liver.
    • Plasma NOx levels decreased by 20% in diabetic rats, and BUN levels were elevated, suggesting kidney damage.

    Conclusions:

    • Diabetes significantly enhances lipid peroxidation, leading to increased oxysterol accumulation in tissues.
    • Diabetes attenuates nitric oxide (NO) generation, likely due to increased oxidative stress and peroxynitrite formation.
    • The kidney is identified as a predominant target organ for early diabetic complications, evidenced by elevated oxysterols and BUN.