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Updated: Aug 18, 2026

Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Published on: September 30, 2015
[The evolutionary traits of mitocondrial ND4-ND4L in Drosophila melanogaster species group]
Shao Liang Mou1, Qing Tao Zeng, Yuan Huai Qian
1College of Life Science, Hubei University, Wuhan, 430062, China.
Abstract:
The sequences of the mitochondrial ND4-ND4L were determined for all the 33 extant taxaes of the Drosophila melanogaster species group, so evolutionary traits of ND4 gene sequences were analyzed. We found a strong transition bias in closed related species and saturation of transitional substitutions in distant related species. Moreover, we calculated the diverge time of eight subgroup according to the Tamura-Nei distance between D. melanogaster and D. yakuba. Our result suggested that ananassae subgroup branched off firstly, and montium, melanogaster, ficsphila, eugracilis, elegans, suzukii-takahashii branched off sequentially.
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