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Determination of oxycodone and hydrocotarnine in cancer patient serum by high-performance liquid chromatography with
Hideya Kokubun1, Makiko Ouki, Motohiro Matoba
1Department of Pharmacy, Kitasato University Hospital, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan. kokubun@nc.kitasato-u.ac.jp
Abstract:
We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotarnine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotarnine were less than 4.8%. The method developed here was better than the method reported previously.
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01:15High-Performance Liquid Chromatography: Types of Detectors
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In HPLC, two phases play a critical role in the separation process: