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Updated: Aug 18, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
DNA sequence-based tandem repeat analysis of the clfB gene is less discriminatory than spa typing for
Dag Harmsen1, Heike Claus, Ulrich Vogel
1Institute for Hygiene, University of Münster, Münster, Germany.
Abstract:
Methicillin-resistant Staphylococcus aureus (MRSA) are a major challenge for hospital hygiene. Typing by DNA sequence analysis of the repeat region of the protein A gene (spa) significantly improved typing of MRSA in the hospital setting. However, microevolution of spa repeats in epidemic clones appears to occur at a fairly slow clock rate. Therefore, DNA sequence-based methods providing additional resolution are desirable in some situations. We evaluated the use of the clfB repeat region proposed recently by others as a possible complementation to spa typing. Using epidemic MRSA isolates from two German university hospitals, we show that the clfB repeat region does not offer any additional discriminatory power.
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