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Updated: Aug 18, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics
D F Harney1, R K Butler, R J Edwards
1Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, 123 St, Stephens Green, Dublin 2, Ireland. dharney@rcsi.ie
Background:
Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site(s) for this phosphorylation event.
Results:
A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation.
Conclusion:
Our work is suggestive that tyrosine phosphorylation of myosin heavy chain, whether in skeletal muscle or in platelets, is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells.
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