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Related Experiment Videos

Stacking due to ionic transport number mismatch during sample sweeping on microchips.

Yingjie Liu1, Robert S Foote, Stephen C Jacobson

  • 1Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6142, USA.

Lab on a Chip
|March 26, 2005
PubMed
Summary

Sample stacking in electrophoresis buffers enhances signal detection. This phenomenon, observed using fluorescence imaging, significantly amplifies signals for hydrophobic dyes, improving analytical sensitivity.

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Electrophoresis

Background:

  • Sample stacking can occur in isoconductive buffer systems due to ion transport mismatches.
  • Changes in buffer conductivity during electrophoresis can lead to undesirable sample dispersion.
  • Hydrophobic dyes are often analyzed using techniques like sweeping with sodium dodecyl sulfate (SDS).

Purpose of the Study:

  • To investigate sample stacking in isoconductive buffer systems using fluorescence imaging.
  • To examine the sweeping of hydrophobic dyes with SDS on microchips.
  • To quantify signal enhancement achieved through sweeping and stacking.

Main Methods:

  • Utilized fluorescence imaging on microchips to observe sample stacking.
  • Employed isoconductive buffer systems, specifically a sodium borate buffer.

Related Experiment Videos

  • Analyzed the sweeping of hydrophobic dyes (Rhodamine 560, Rhodamine B, Rhodamine 6G) with sodium dodecyl sulfate (SDS).

    • Main Results:

    • Observed sample stacking in an isoconductive sodium borate buffer system.
    • Demonstrated that SDS micelles sweep sample plugs, leading to stacking at the trailing end.
    • Achieved significant signal enhancements (86- to 560-fold) for Rhodamine dyes via sweeping and stacking.

    Conclusions:

    • Sample stacking in isoconductive buffers is a viable method for signal enhancement in microchip electrophoresis.
    • The observed stacking effect can be modeled using moving boundary equations.
    • Developed a method for analyte trapping and concentration from multiple injections using sweeping/stacking and electric field manipulation.