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Updated: Jul 15, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Cofactor regeneration at the lab scale.

R Wichmann¹, D Vasic-Racki

¹Biochemical Engineering Group, Department of Biochemical and Chemical Engineering, Universität Dortmund, 44221 Dortmund, Germany. wichmann@bci.uni-dortmund.de

Advances in Biochemical Engineering/Biotechnology

|March 29, 2005

Summary

Lab-scale coenzyme regeneration systems, particularly for NAD+/NADH and NADP+/NADPH, have advanced significantly. Various in situ methods, including enzymatic and electrochemical approaches, show promise for efficient biocatalysis.

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Area of Science:

Biotechnology
Biocatalysis
Enzyme Engineering

Background:

Coenzymes like NAD+/NADH and NADP+/NADPH are crucial for redox reactions in biological systems.
Efficient regeneration of these coenzymes is essential for cost-effective biocatalytic processes.
Lab-scale advancements in coenzyme regeneration have been a focus for two decades.

Purpose of the Study:

To review progress in lab-scale coenzyme regeneration systems.
To highlight NAD+/NADH and NADP+/NADPH-dependent oxidoreductase reactions.
To discuss various in situ regeneration strategies and enzyme reaction engineering.

Main Methods:

Review of in situ regeneration systems: whole cell, enzymatic, electro-enzymatic, chemical, and photochemical.
Analysis of efficiency and novelty of presented methods.
Reporting on progress in enzyme reaction engineering.

Main Results:

Significant progress in lab-scale coenzyme regeneration systems over the past 20 years.
Focus on NAD+/NADH and NADP+/NADPH-dependent oxidoreductase reactions.
Development and evaluation of diverse in situ regeneration techniques.

Conclusions:

Various coenzyme regeneration systems have been developed and assessed for lab-scale applications.
Enzymatic and electro-enzymatic methods show particular promise for efficient NAD+/NADH and NADP+/NADPH regeneration.
Continued progress in enzyme reaction engineering is vital for optimizing these systems.