Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhanced ALA-induced fluorescence in hyperparathyroidism.

Ruediger L Prosst1, Lioba Schroeter, Johannes Gahlen

  • 1Department of Surgery, University Hospital Mannheim, Ruprecht-Karls-University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. ruediger@prosst.de

Journal of Photochemistry and Photobiology. B, Biology
|March 29, 2005
PubMed
Summary

Hyperparathyroidism significantly enhances aminolevulinic acid (ALA)-induced fluorescence in parathyroid glands. This fluorescence difference could enable safer, minimally invasive surgery for parathyroid diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Undifferentiated Intimal Sarcoma of the Large Abdominal Arteries Presenting As Acute Mesenteric Ischemia: A Case Report.

Cureus·2026
Same author

The mechanosensitive channel ELKIN1 regulates cellular adaptations to simulated microgravity.

NPJ microgravity·2025
Same author

Modulating the Mechanical Activation of TRPV4 at the Cell-Substrate Interface.

Frontiers in bioengineering and biotechnology·2021
Same author

TMEM87a/Elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration.

eLife·2020
Same author

Pre-clinical study on a telemetric gastric sensor for recognition of acute upper gastrointestinal bleeding: the "HemoPill monitor".

Surgical endoscopy·2019
Same author

A randomized comparative trial of OTSC and Padlock for upper GI hemostasis in a standardized experimental setting.

Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy·2017

Area of Science:

  • Biomedical Engineering
  • Surgical Oncology
  • Endocrinology

Background:

  • Intraoperative parathyroid gland localization is difficult, particularly in minimally invasive surgery.
  • Aminolevulinic acid (ALA) fluorescence diagnosis has shown potential for identifying normal parathyroid glands.
  • This study investigates fluorescence differences between hyperplastic and normal parathyroid glands for clinical application.

Purpose of the Study:

  • To evaluate the fluorescence characteristics of hyperplastic versus normal parathyroid glands using ALA.
  • To determine if ALA-induced fluorescence can differentiate parathyroid glands in hyperparathyroidism.
  • To establish a precondition for clinical application of ALA fluorescence in parathyroid surgery.

Main Methods:

  • Polycystic kidney disease (PKD) rats (hyperplastic glands) and Wistar rats (normal glands) were administered ALA.

Related Experiment Videos

  • Fluorescence of parathyroid and thyroid glands was assessed under blue light (d-light system).
  • Spectrometry measured fluorescence intensity; serum parathyroid hormone levels were determined by ELISA.

    • Main Results:

    • PKD rats exhibited stronger red fluorescence in parathyroid glands compared to Wistar rats.
    • Spectrometry showed 10x higher fluorescence in PKD rat parathyroid glands vs. thyroid, versus 3.2:1 in Wistar rats.
    • Parathyroid glands in PKD rats were over twice as fluorescent as in Wistar rats; ELISA confirmed hyperparathyroidism.

    Conclusions:

    • Hyperparathyroidism intensifies ALA-induced fluorescence in parathyroid glands.
    • This enhanced fluorescence may support unilateral, minimally invasive approaches in parathyroid surgery.
    • The technique could improve surgeon's ability to manage parathyroid diseases safely and efficiently.