Sensititre YeastOne caspofungin susceptibility testing of Candida clinical isolates: correlation with results of NCCLS M27-A2 multicenter study
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Published on: December 28, 2017
Emilia Cantón1, Javier Pemán, Miguel Gobernado
1Unidad de Microbiología Experimental-Centro de Investigación, Hospital Universitario La Fe, Avenida Campanar 21, 46009 Valencia, Spain. canton_emi@gva.es
Abstract:
The ability of Sensititre YeastOne to discriminate isolates with reduced caspofungin susceptibility was determined against 36 Candida spp. (6 with a known FKS1 mutation). Results were compared with those of M27-A2. The MIC endpoint was 100% growth inhibition. Overall agreement (+/-2 log2) was 87.16%. Sensititre YeastOne detected strains with reduced caspofungin susceptibility.
Insights
The Sensititre YeastOne assay effectively identifies Candida species with reduced susceptibility to caspofungin. This method shows good agreement with established standards, aiding in antifungal resistance monitoring.
Area of Science:
- Mycology
- Clinical Microbiology
- Antifungal Drug Resistance
Background:
- Caspofungin is a key antifungal agent used against Candida infections.
- Monitoring antifungal susceptibility is crucial for effective treatment.
- Emerging resistance to echinocandins, including caspofungin, necessitates reliable detection methods.
Purpose of the Study:
- To evaluate the performance of the Sensititre YeastOne system for detecting reduced caspofungin susceptibility in Candida isolates.
- To compare the Sensititre YeastOne results with a reference method (M27-A2).
Main Methods:
- Testing of 36 Candida isolates, including 6 with known FKS1 mutations conferring resistance.
- Determination of Minimum Inhibitory Concentrations (MICs) using Sensititre YeastOne.
- Comparison of results with the M27-A2 standard method, using 100% growth inhibition as the endpoint.
Main Results:
- The Sensititre YeastOne system demonstrated 87.16% agreement (+/-2 log2) with the M27-A2 reference method.
- The assay successfully identified Candida strains exhibiting reduced susceptibility to caspofungin.
- Detection of resistance was correlated with the presence of FKS1 mutations.
Conclusions:
- Sensititre YeastOne is a reliable method for detecting reduced caspofungin susceptibility in Candida species.
- This assay can aid in clinical laboratories for guiding antifungal therapy.
- The findings support the use of Sensititre YeastOne in routine antifungal susceptibility testing.
