Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time intervals (3' or 5') between dose steps can influence methacholine challenge test.

Salvatore Mariotta1, Bruno Sposato, Alberto Ricci

  • 1Dipartimento Scienze Cardiovascolari e Respiratorie, Università La Sapienza, U.O. Pneumologia, Azienda Ospedaliera Sant'Andrea, Via di Grottarossa 1035-39, 00189 Rome, Italy. salvatore.mariotta@uniromal.it

Lung
|March 29, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Lean and Value-Based Healthcare methods to support the chronic heart failure care model.]

Recenti progressi in medicina·2026
Same author

How many blood eosinophil measurements are needed to identify COPD patients eligible for biologic therapy? A multi-source decision analysis simulation.

European journal of internal medicine·2026
Same author

Time-Driven Activity-Based-Costing to Improve Healthcare Resource Use: Patient Pathways and Cost Comparisons for Cardiac Electrophysiology Interventions and Surgical Aortic Valve Replacement in Five Italian Hospitals.

Clinical therapeutics·2026
Same author

No Time to Bleed! Ultrasound-Guided Management of a Pleural Biopsy Complication: A Case Report.

Respirology case reports·2026
Same author

Impact of IL-4/IL-13 Blockade with Dupilumab on the Microbiome in Type 2 Inflammatory Diseases: A Systematic Review.

Current allergy and asthma reports·2026
Same author

Mucus Plugging as a Treatable Trait Across the Asthma-COPD Spectrum: The Role of Type 2 Cytokine Blockade and Quantitative Imaging.

Biomedicines·2026

The time interval between methacholine doses impacts bronchial hyperresponsiveness (BHR) testing. Longer intervals (5 min) may lead to inaccurate BHR assessments in mild and borderline asthmatics compared to shorter 3-min intervals.

Area of Science:

  • Pulmonology
  • Allergy and Immunology
  • Clinical Pharmacology

Background:

  • Bronchial hyperresponsiveness (BHR) is a hallmark of asthma.
  • Methacholine challenge (MCH) is the standard test for BHR assessment.
  • Standardization of MCH protocols, including time intervals, is crucial for accurate diagnosis.

Purpose of the Study:

  • To investigate the influence of 3-minute (MCH-3') versus 5-minute (MCH-5') intervals between methacholine doses on BHR classification.
  • To determine if different time intervals affect the estimation of BHR severity in asthmatic subjects.

Main Methods:

  • A cohort of 52 intermittent asthmatics underwent two MCH tests with different dose intervals (3 min and 5 min).
  • Subjects were classified into moderate (BHR-M), mild (BHR-m), and borderline (BHR-B) BHR categories based on MCH-3' results.

Related Experiment Videos

  • Cumulative doses, PD20FEV(1), and dose-response slopes were compared between MCH-3' and MCH-5' protocols.

    • Main Results:

    • MCH-5' resulted in higher cumulative doses and PD20FEV(1) compared to MCH-3' in BHR-m and BHR-B groups (p < 0.05).
    • Dose-response slopes differed significantly between MCH-3' and MCH-5' in BHR-m (p < 0.05) and BHR-B (p < 0.01) groups.
    • MCH-5' identified a higher number of subjects with normal reactivity compared to MCH-3'.

    Conclusions:

    • The 5-minute interval may lead to an underestimation of BHR severity in mild and borderline asthmatics due to incomplete drug metabolism.
    • Standardizing the time interval between methacholine doses is essential for improving the comparability and reliability of BHR testing.
    • Further research is needed to establish optimal MCH protocols for accurate BHR assessment.