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Related Experiment Videos

Liquid chromatographic method for quantifying polyphenols in ciders by direct injection.

Belén Suárez1, Noemí Palacios, Natalia Fraga

  • 1Area de Tecnología de los Alimentos, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), 33300-Villaviciosa, Asturias, Spain.

Journal of Chromatography. A
|March 30, 2005
PubMed
Summary

A new method accurately quantifies phenolic compounds in cider using RP-HPLC without sample prep. This analysis aids in establishing detailed phenolic profiles for ciders.

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Area of Science:

  • Analytical Chemistry
  • Food Chemistry

Background:

  • Phenolic compounds are crucial for cider quality and health benefits.
  • Accurate quantification of these compounds is essential for quality control and understanding cider's properties.

Purpose of the Study:

  • To develop and validate a rapid analytical method for determining key phenolic compounds in cider.
  • To establish a phenolic profile for Asturian cider using the developed method.

Main Methods:

  • Reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with a photodiode-array (PDA) detection system.
  • Direct analysis of cider samples without prior sample treatment.
  • Comprehensive method validation including linearity, detection limits, recovery, and reproducibility.

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Main Results:

  • The method demonstrated excellent linearity (correlation coefficients > 0.999).
  • Low detection limits were achieved, ranging from 0.07 mg/L to 2 mg/L.
  • High recoveries (90–104%) and good reproducibility (< 8% RSD) were confirmed.
  • The method was successfully applied to commercial cider samples.

Conclusions:

  • A robust and efficient RP-HPLC-PDA method for phenolic compound analysis in cider has been established.
  • The validated method provides a reliable tool for quality assessment and profiling of cider.
  • The findings contribute to understanding the phenolic composition of Asturian cider.