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Training of yeast cell dynamics.

Karin A Reijenga1, Barbara M Bakker, Coen C van der Weijden

  • 1Department of Molecular Cell Physiology, CRbCS, BioCentrum Amsterdam, Faculty of Earth and Life Sciences, Vrije Universiteit, Amsterdam, Netherlands.

The FEBS Journal
|March 30, 2005
PubMed
Summary

Yeast cells adapt to fluctuating glucose levels by increasing their internal response dynamics. This "training" involves enhanced glucose transport affinity, crucial for adapting to environmental changes in fermentation and natural settings.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Cellular Dynamics

Background:

  • Microorganisms in industrial fermenters and natural environments face fluctuating conditions.
  • Understanding cellular responses to dynamic environments is key for optimizing biotechnological processes.

Purpose of the Study:

  • To investigate how yeast cells adapt their intracellular dynamics to oscillating extracellular glucose concentrations.
  • To elucidate the mechanism behind cellular adaptation, termed 'training', to fluctuating nutrient availability.

Main Methods:

  • Mimicking fluctuating glucose levels by imposing oscillating extracellular glucose concentrations on yeast cell suspensions.
  • Monitoring intracellular dynamics via NADH fluorescence.
  • Assessing changes in glucose transporter kinetics (K(M)) and the effect of maltose.

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Main Results:

  • Extracellular glucose oscillations induced dynamic intracellular NADH fluorescence in yeast cells.
  • The amplitude of NADH oscillations increased over time, indicating cellular adaptation or 'training'.
  • Adaptation to low, constant glucose also increased NADH oscillation amplitude, and decreased glucose transporter K(M) from 26 mM to 3.5 mM.

Conclusions:

  • Yeast cells exhibit a 'training' response to fluctuating glucose, enhancing their intracellular dynamics.
  • This training is mediated by an increased affinity of the glucose transporter, essential for adapting to dynamic nutrient environments.
  • Cellular adaptation, rather than medium changes, drives the enhanced response to environmental fluctuations.