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Related Experiment Videos

Diagnostic tests for small ruminant lentiviruses.

D de Andrés1, D Klein, N J Watt

  • 1Institute of Agrobiotechnology and Natural Resources, Pamplona, Spain.

Veterinary Microbiology
|March 30, 2005
PubMed
Summary

Maedi Visna Virus (MVV) and Caprine Arthritis Encephalitis Virus (CAEV) cause slow-progressing diseases in small ruminants. Combining ELISA and PCR offers the best detection for these retroviral infections.

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Small ruminant lentivirus-induced arthritis: clinicopathologic findings in sheep infected by a highly replicative SRLV B2 genotype.

Veterinary pathology·2014

Area of Science:

  • Veterinary Virology
  • Immunology
  • Molecular Diagnostics

Background:

  • Maedi Visna Virus (MVV) and Caprine Arthritis Encephalitis Virus (CAEV) are closely related retroviruses.
  • These viruses cause chronic, slow-progressing inflammatory diseases in small ruminants.
  • Current diagnosis relies on serological testing, but assay performance varies.

Purpose of the Study:

  • To review recent advancements in laboratory diagnostic methods for SRLV.
  • To evaluate the use of these methods in field diagnosis.
  • To compare the sensitivity and specificity of available diagnostic assays.

Main Methods:

  • Systematic review of recent literature on diagnostic methods for SRLV.
  • Analysis of laboratory diagnostic techniques including ELISA and PCR.

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  • Evaluation of field applicability of diagnostic assays.

    • Main Results:

    • A variety of serological assays exist for SRLV detection.
    • Relative sensitivity and specificity of these assays have not been systematically compared.
    • ELISA and PCR show promise for optimal SRLV detection.

    Conclusions:

    • Optimal detection of Small Ruminant Lentivirus (SRLV) infection may be achieved by combining ELISA and PCR.
    • Further systematic comparisons of diagnostic assays are needed.
    • Advancements in laboratory diagnostics can improve field diagnosis of SRLV.