Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-resolution functional proteomics by active-site peptide profiling.

Eric S Okerberg1, Jiangyue Wu, Baohong Zhang

  • 1ActivX Biosciences, 11025 North Torrey Pines Road, Suite 120, La Jolla, CA 92037, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 30, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomic reconstruction of upland cotton domestication uncovers staged selection, gene flow, and flowering-time adaptation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Heterophyllin B ameliorates diabetic lower limb ischemia by inhibiting SMOX to activate the Nrf2 antioxidant pathway.

Chinese medicine·2026
Same author

Depression and anxiety changes in patients after orthognathic surgery: a systematic review and meta-analysis.

The British journal of oral & maxillofacial surgery·2026
Same author

Utilizing Environmental DNA Metabarcoding to Assess Fish-Based Food Resources in Key Foraging Areas of the Black Stork.

Ecology and evolution·2026
Same author

SpliceHarmonization: an integrated method for identifying RNA splicing events in therapeutics for splicing modulation.

Bioinformatics (Oxford, England)·2026
Same author

Normalizing Covalent Potency for Electrophilicity with Ligand Reactivity Efficiency.

Journal of medicinal chemistry·2026
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

This study introduces a new proteomics platform for analyzing protein activities, enabling the discovery of disease-related enzymes and potential drug inhibitors in complex biological samples.

Area of Science:

  • Proteomics
  • Biochemistry
  • Enzymology

Background:

  • Genome sequencing yields numerous proteins requiring functional characterization.
  • Existing proteomics methods primarily quantify protein abundance, not activity.
  • Analyzing protein function in complex samples like plasma is challenging.

Purpose of the Study:

  • To present a novel gel-free platform for analyzing proteome-wide protein activities.
  • To enable ultrasensitive, quantitative, and high-resolution functional proteomic analysis.
  • To demonstrate the platform's utility in discovering disease-related enzyme activities and inhibitors.

Main Methods:

  • Development of a gel-free, high-resolution proteomics platform.
  • Application of the platform to analyze protein activities in various proteomes.

Related Experiment Videos

  • Utilizing the platform for discovery of enzyme activities and inhibitor screening.

    • Main Results:

    • The platform provides ultrasensitive and quantitative analysis of protein activities.
    • Successfully analyzed complex and challenging samples, including undiluted plasma.
    • Demonstrated the discovery of disease-associated enzyme activities.
    • Identified specific inhibitors targeting disease-related enzymes.

    Conclusions:

    • The developed platform addresses a critical gap in functional proteomics.
    • It enables sensitive discovery of enzyme activities and drug targets in complex biological systems.
    • This technology has significant implications for disease research and drug development.