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Updated: Aug 18, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Acetylecholinesterase-based biosensor electrodes for organophosphate pesticide detection. II. Immobilization and
A Vakurov1, C E Simpson, C L Daly
1School of Biochemistry and Microbiology, University of Leeds, Leeds, LS2 9JT, UK.
Abstract:
The dry and wet stability of Drosophila acetylcholinesterase non-covalently immobilized onto polyethyleneimine modified screen-printed carbon electrodes was improved when compared to non-immobilized acetylcholinesterase, and acetylcholinesterase covalently immobilized onto dialdehyde and polyethyleneimine modified electrodes. Stabilizer mixtures were characterized for additional stabilization of acetylcholinesterase during storage in the dry state, with dextran-sulphate/sucrose and polygalacturonic acid/sucrose mixtures proving highly effective for long-term storage of biosensor electrodes.
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