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Updated: Aug 18, 2026
Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
Published on: September 19, 2014
Detection of single and multiple targets in tissue phantoms with fluorescence-enhanced optical imaging: feasibility
Anuradha Godavarty1, Margaret J Eppstein, Chaoyang Zhang
1Photon Migration Laboratories, Texas A&M University, PO Box 30012, College Station, TX 77842-3012, USA.
Purpose:
To evaluate the feasibility of reconstructing single and multiple targets by using fluorescence-enhanced tomography of a breast tissue phantom.
Materials And Methods:
Frequency-domain fluorescence measurements were performed in cup-shaped tissue phantoms of clinically relevant size (diameter, 10 cm) in which single or multiple 0.5-1.0-cm(3) targets that contained micromolar concentrations of indocyanine green with 1:0 and 100:1 target-to-background (T:B) contrast ratios had been embedded. Rapid acquisition of time-dependent fluorescent light measurements was performed at the phantom surface in response to point illumination of excitation light by using a gain-modulated intensified charge-coupled device detection system. Boundary surface measurements were used to tomographically reconstruct the interior targets located with various experimental conditions.
Results:
Single 1.0-cm(3) targets located between 1.43 and 2.82 cm deep from the phantom surface at a T:B contrast ratio of 100:1 and three approximately 0.55-cm(3) targets located about 1.30 cm deep at a T:B contrast ratio of 1:0 were reconstructed with minimal or no artifacts by using boundary surface fluorescence measurements and an approximate extended Kalman filter algorithm.
Conclusion:
It is feasible to detect single or multiple fluorescent targets in tissue phantoms of clinically relevant size by using fluorescence-enhanced optical tomography.
