Related Experiment Video
Updated: Aug 18, 2026
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
Published on: September 15, 2014
BG01V: a variant human embryonic stem cell line which exhibits rapid growth after passaging and reliable dopaminergic
Xianmin Zeng1, Jia Chen, Ying Liu
1Cellular Neurobiology Branch, National Institute on Drug Abuse and NIH, DHHS, Baltimore, MD 21224, USA. xzeng@mail.nih.gov
Purpose:
To explore a karyotypically abnormal variant human embryonic stem cell (hESC) line, BG01V, as a potential model for studies of dopaminergic neuronal differentiation.
Methods:
The properties of BG01V cells were compared to those of normal BG01 cells using immunocytochemistry, RT-PCR, focused microarrays and in vitro differentiation, including dopaminergic differentiation, by culturing with the stromal cell line PA6.
Results:
Despite the karyotypic abnormality (49, +12, +17 and XXY), undifferentiated BG01V cells expressed pluripotent ESC markers similar to BG01 cells, and retained the ability to differentiate into cell types characteristic of all three germ layers. When co-cultured with the stromal cell line PA6, BG01V cells differentiated into dopaminergic cells which exhibited properties similar to those of mature dopaminergic neurons.
Conclusions:
BG01V cells were easier to maintain in culture than karyotypically normal BG01 cells and can be used as an alternative pluripotent hESC type for in vitro developmental studies.
More Related Videos
11:17Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
Published on: January 18, 2020
12:13Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
Published on: July 11, 2019
