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Escape of phagocytosis-activating function from platelets into plasma during storage
1Department of Internal Medicine, Wakayama Medical College, Japan.
Vox Sanguinis
|January 1, 1992
Summary
During storage, phagocytosis-enhancing glycoproteins (macromolecular activators of phagocytosis, MAPP) are released from platelets into plasma. Suppressing activity, however, remains within platelets for at least 48 hours.
Area of Science:
- Immunology
- Hematology
- Biochemistry
Background:
- Leukocyte phagocytosis is crucial for immune response.
- Platelet concentrate storage can alter its biological properties.
- Platelet release products (PRPr) contain various bioactive molecules.
Purpose of the Study:
- To investigate the fate of phagocytosis-modulating glycoproteins during platelet concentrate storage.
- To characterize the phagocytosis-enhancing and suppressing activities in stored platelets.
Main Methods:
- Utilized stored platelet concentrate (PC) and its supernatant (Sup).
- Employed ammonium sulfate (AS) precipitation and Sephadex G200 gel filtration.
- Assessed leukocyte phagocytic activity and PRPr composition.
Main Results:
- Phagocytosis-enhancing activity (l-MAPP and s-MAPP) was found in the supernatant of PC stored >48h, not in the platelets themselves.
- Phagocytosis-suppressing activity was present in PRPr from both fresh and stored PC.
- Gel filtration revealed l-MAPP and s-MAPP in the supernatant matched molecular weights found in fresh PRPr.
Conclusions:
- l-MAPP and s-MAPP are released from platelets into plasma during storage.
- Platelet-retained phagocytosis suppression persists for at least 48 hours.
- Storage duration impacts the distribution of MAPP and suppressing factors in platelet products.